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Objective:This study conducted a statistical analysis of the 2010 to 2019 Chinese Medical Science and Technology Award to understand its current situation and research hotspots, to provide references for medical science and technology investigators and administrators.Methods:Through the official website of the Chinese Medical Association, the 2010 to 2019 Chinese Medical Science and Technology Award data was collected. Information includes gender of the awardees, award level, geographical distribution, discipline distribution, and completion unit of the first person was analyzed. Network metrology, social network analysis and word cloud graph analysis are used to analyze the " development status" , " cooperative relationship" and " research hotspots" of medical research in China.Results:The total number of winning projects was 857. Among them, the number of first, second, and third prizes were 76, 249 and 475. Beijing is the area with the largest number of winners (277), followed by Shanghai (167) and Jiangsu (92). Among the winning projects, the top 3 subject were oncology, cardiology, orthopaedics, general surgery with percentages accounted by 9.78%, 7.94%, 6.21%.Conclusions:The geographical distribution of winning projects presented a regional concentration trend. The disciplines of award-winning projects have been diversified, especially in oncology. The gender ratio of principal investigators is uneven, most were male. Future research should pay more attention to multidisciplinary, cross-regional facilitation, and promote the coordinated development of holistic medicine.
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Objective This paper selects indicators,such as SCI journal publication fee,impact factors,annual Web of Science Documents,volume of publications in China,articles cited and so on,to conduct the feasibility analysis of using publication fee /impact factor (BI) as evaluation index of SCI journals.Methods Information from Web of Science,InCites,Journal Citation Reports database and the real-time data of 2013-2017 years SCI publication fees in Central South University,the third Xiangya Hospital were collected,SPSS and EXCEL was used to conduct Correlation and Basic analysis.Results In 2013-2017,a total number of 31 kinds of SCI journals were included in sample size,the SCI journal impact factor was positively correlated with the publication fee.BI was negative correlated with IF,The publication fee was not related to annual Web of Science Documents,volume of publications in Mainland China and Cites/Paper.Conclusions The SCI journal publication fee/impact factor (BI) can be used as a macro evaluation index for SCI journals.The BI values also can provide reference and reference of decision support and journal selection for scientific research personnel and scientific research administrators.
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Stable isotope coded derivatization (ICD) is an isotope labeling technique for specific functional groups of the target analytes through chemical derivatization.ICD combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) enables systematically analyzing the biomolecules with the same labeling reaction characteristic.ICD technique can effectively solve the limited sensitivity in complex bio-matrices analysis and unavailability of isotope internal standards in quantitative analysis.In recent years, ICD technique has been widely used in metabolomics research field.Based on this background, the ICD technique and the design of ICD reagents are briefly described in this review.The recent advances in ICD reagents for carboxyl, amino, carbonyl, thiol and hydroxyl groups and their applications in the analysis of small molecule metabolites in bio-matrices with LC-MS/MS are reviewed.
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Since γH2 AX was firstly found in 1998 , it has been one of the most important scientific topics and research tools in the related scientific fields. At present, a series of advanced testing methods and analytical technologies have been developed, which exhibited a quite attractive application prospect in the area of life science and medical science. This paper reviewed the latest progress about γH2AX in terms of molecular mechanism of phosphorylation/dephosphorylation, development of testing technologies, and the related applications.
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Phospho-histone H2AX(γH2AX) has been widely used in vitro genotoxicity evaluation of ionizing radiation, carcinogenic substances and cigarette smoke as an important biomarker of DNA double stranded breaks ( DSBs) . The study developed an enzyme-linked immunesorbent assay for detection of the content ofγH2AX in cells to evaluate the genotoxicity of cigarette smoke. The study exposed CHO cells with cigarette smoke total particulate matter ( TPM) and cigarette smoke condensate ( CSC) of different dose, dose-effect relationship between smoke exposure and DNA damage and the poisonous difference of cigarette smoke components has been investigated by detecting the content change ofγH2 AX in cells under different exposure time and dose. In addition, reactive oxygen species ( ROS) in cells were also detected to study the mechanism of cigarette smoke exposure induced DSBs. The experiment result showed that the required time for the level ofγH2AX in cells grew to the peak prolonged with increased exposure dose. Once the level of γH2AX in cells grew to the peak value, then it decreased slowly; the level of γH2AX in cells rised with increasing dose of cigarette smoke;The effect of TPM on the content change of γH2AX was more than CSC; Moreover, smoke exposure could induce concentration increase of ROS in cells, and a good correlation of content change of ROS and γH2AX in cells were found, free radicals in cigarette smoke may be one major cause of DSBs.
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A liquid chromatography-tandem mass spectrometry ( LC-MSMS ) method has been developed for the simultaneous determination of N3-methyladenine ( N3-MeA ) and N3-ethyladenine ( N3-EtA ) in calf thymus DNA. The DNA samples has been purified and enriched by cation exchange cartridge ( Waters Oasis MCX) . d3-N3-MeA and d5-N3-EtA were used as isotope internal standard. The DNA samples were injected with autosampler. The injected volume was 3 μL and analysis time was 13 min. The sample separation was carried out on hydrophilic interaction chromatograph ( Waters XBridge HILIC ) with 10 mmol/L ammonium formate-acetonitrile (5:92, V/V, pH=4. 0) as mobile phase. The flow rate was set at 250 μL/min. Mass spectrometry was performed by electrospray ionization ( ESI ) with multi-reactions monitoring ( MRM ) . The optimized operation conditions of MS were as follows: nebulizer gas 369 Pa; curtain gas 185 Pa, turbo ionspray temperature 400 ℃, ionspray voltage 5500 V, dwell time 40 ms. The limits of detection were 0. 043 and 0. 007 μg/L for N3-MeA and N3-EtA, respectively. The recoveries were between 87. 8% and 103. 0%for N3-MeA and N3-EtA. This method was successfully applied to the determination of N3-MeA and N3-EtA in calf thymus DNA by cigarette smoke condensate ( CSC) exposure. This method is appropriate for routine analysis and accurate quantification of N3-MeA and N3-EtA by CSC exposure.